Improvement of the Lipophilic-Oxygen Radical Absorbance Capacity (L-ORAC) Method and Single-Laboratory Validation

Jun WATANABE; Tomoyuki OKI; Jun TAKEBAYASHI; Koji YAMASAKI; Yuko TAKANO-ISHIKAWA; Akihiro HINO; Akemi YASUI

doi:10.1271/bbb.120786

Food & Nutrition Science Notes

Improvement of the Lipophilic-Oxygen Radical Absorbance Capacity (L-ORAC) Method and Single-Laboratory Validation

Jun WATANABE, Tomoyuki OKI, Jun TAKEBAYASHI, Koji YAMASAKI, Yuko TAKANO-ISHIKAWA, Akihiro HINO, Akemi YASUI

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Keywords: lipophilic oxygen radical absorbance capacity, antioxidant capacity, method validation

JOURNAL FREE ACCESS

2013 Volume 77 Issue 4 Pages 857-859

DOI https://doi.org/10.1271/bbb.120786

View "Advance Publication" version (April 7, 2013).

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Abstract

We improved the procedure for lipophilic-oxygen radical absorbance capacity (L-ORAC) measurement for better repeatability and intermediate precision. A sealing film was placed on the assay plate, and glass vials and microdispensers equipped with glass capillaries were used. The antioxidant capacities of food extracts can be evaluated by this method with nearly the same precision as antioxidant solutions.

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