Molecular Cloning and Sequence Analysis of an Extracellular Protease from Four Bacillus subtilis Strains

Xujun HAN; Yuh SHIWA; Masanori ITOH; Tohru SUZUKI; Hirofumi YOSHIKAWA; Toshiyuki NAKAGAWA; Hiroko NAGANO

doi:10.1271/bbb.120920

Biochemistry & Molecular Biology Notes

Molecular Cloning and Sequence Analysis of an Extracellular Protease from Four Bacillus subtilis Strains

Xujun HAN, Yuh SHIWA, Masanori ITOH, Tohru SUZUKI, Hirofumi YOSHIKAWA, Toshiyuki NAKAGAWA, Hiroko NAGANO

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Keywords: Bacillus subtilis, alkaline protease, clone, activity

JOURNAL FREE ACCESS

2013 Volume 77 Issue 4 Pages 870-873

DOI https://doi.org/10.1271/bbb.120920

View "Advance Publication" version (April 7, 2013).

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Abstract

Four Bacillus subtilis strains were isolated from traditional fermented foods, and the sequences of their extracellular alkaline proteases (AprE) were analyzed and cloned. The recombinant enzymes synthesized by means of Escherichia coli exhibited high proteolytic activity. AprE CN2 showed hydrolytic activity 4-fold higher than that of AprE 168. This activity was also seen in the presence of relatively high NaCl concentrations.

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