Microbiology & Fermentation Technology Note
Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation
2013 年 77 巻 4 号 p. 874-876
詳細
2013 年 77 巻 4 号 p. 874-876
A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without removing rigid silica-based cell walls, and high transformation efficiency (about 4,500 per 108 cells) is achieved.
この記事は最新の被引用情報を取得できません。
