Improvement of Ethanol Production from <small>D</small>-Lactic Acid by Constitutive Expression of Lactate Transporter Jen1p in Saccharomyces cerevisiae

Makoto WAKAMATSU; Masataka TOMITAKA; Tatsunori TANI; Hisataka TAGUCHI; Kenji KIDA; Takashi AKAMATSU

doi:10.1271/bbb.120985

Microbiology & Fermentation Technology Notes

Improvement of Ethanol Production from D-Lactic Acid by Constitutive Expression of Lactate Transporter Jen1p in Saccharomyces cerevisiae

Makoto WAKAMATSU, Masataka TOMITAKA, Tatsunori TANI, Hisataka TAGUCHI, Kenji KIDA, Takashi AKAMATSU

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Keywords: D-lactic acid, monocarboxylate/proton symporter gene (JEN1), ethanol, Saccharomyces cerevisiae

JOURNAL FREE ACCESS

2013 Volume 77 Issue 5 Pages 1114-1116

DOI https://doi.org/10.1271/bbb.120985

View "Advance Publication" version (May 7, 2013).

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Abstract

To improve ethanol production from D-lactate, Jen1p, a monocarboxylate-proton symporter, was constitutively expressed in Saccharomyces cerevisiae NAM34-4C. The mutant produced 2.4 g/L of ethanol, approximately 2.4 times higher than that of the wild-type strain. A monocarboxylate/proton symporter gene (JEN1) null mutant was also constructed. It produced 0.19 g/L of ethanol, 5 times lower than that of the wild-type strain.

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