Styrene Altered Clock Gene Expression in Serum-Shocked Cultured Human Fibroblasts

Nicola MANZELLA; Massimo BRACCI; Sara STAFFOLANI; Elisabetta STRAFELLA; Venerando RAPISARDA; Matteo VALENTINO; Monica AMATI; Alfredo COPERTARO; Lory SANTARELLI

doi:10.1271/bbb.120944

Environmental Science Note

Styrene Altered Clock Gene Expression in Serum-Shocked Cultured Human Fibroblasts

Nicola MANZELLA, Massimo BRACCI, Sara STAFFOLANI, Elisabetta STRAFELLA, Venerando RAPISARDA, Matteo VALENTINO, Monica AMATI, Alfredo COPERTARO, Lory SANTARELLI

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Keywords: circadian rhythm, biological clock, xenobiotics, styrene, fibroblast

JOURNAL FREE ACCESS

2013 Volume 77 Issue 6 Pages 1296-1298

DOI https://doi.org/10.1271/bbb.120944

View "Advance Publication" version (June 7, 2013).

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Abstract

The circadian clock can regulate the metabolic process of xenobiotics, but little is known as to circadian rhythms can be perturbed by xenobiotics. Styrene is a organic chemical widely used in occupational settings. The effects of styrene on the circadian genes of HuDE cells were evaluated after serum-shocking synchronization. A subtoxic dose of 100 µM of styrene altered the expression of clock genes BMAL1, PER2, PER3, CRY1, CRY2, and REV-ERB-α.

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