Quantification of Major Royal Jelly Protein 1 in Fresh Royal Jelly by Indirect Enzyme-Linked Immunosorbent Assay

Kikuji YAMAGUCHI; Shaoyu HE; Zhengyue LI; Kiyoshi MURATA; Nobuyuki HITOMI; Manaho MOZUMI; Risa ARIGA; Toshiki ENOMOTO

doi:10.1271/bbb.130013

Food & Nutrition Science Notes

Quantification of Major Royal Jelly Protein 1 in Fresh Royal Jelly by Indirect Enzyme-Linked Immunosorbent Assay

Kikuji YAMAGUCHI, Shaoyu HE, Zhengyue LI, Kiyoshi MURATA, Nobuyuki HITOMI, Manaho MOZUMI, Risa ARIGA, Toshiki ENOMOTO

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Keywords: royal jelly, MRJP1, apisin, ELISA, quantification

JOURNAL FREE ACCESS

2013 Volume 77 Issue 6 Pages 1310-1312

DOI https://doi.org/10.1271/bbb.130013

View "Advance Publication" version (June 7, 2013).

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Abstract

Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12–4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.

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