Establishment of a New Detection System for the Dimerization of IRE1α by BiFC Assay

Satoko SHINJO; Etsu TASHIRO; Masaya IMOTO

doi:10.1271/bbb.130107

Biochemistry & Molecular Biology Notes

Establishment of a New Detection System for the Dimerization of IRE1α by BiFC Assay

Satoko SHINJO, Etsu TASHIRO, Masaya IMOTO

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Keywords: inositol requiring kinase 1 α (IRE1α), imaging, bimolecular fluorescent complementation (BiFC) assay, unfolded protein response (UPR), small molecule

JOURNAL FREE ACCESS

2013 Volume 77 Issue 6 Pages 1333-1336

DOI https://doi.org/10.1271/bbb.130107

View "Advance Publication" version (June 7, 2013).

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Abstract

We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.

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