Production of Hydroxlated Flavonoids with Cytochrome P450 BM3 Variant F87V and Their Antioxidative Activities

Emi KITAMURA; Toshihiko OTOMATSU; Chiemi MAEDA; Yoko AOKI; Chihiro OTA; Norihiko MISAWA; Kazutoshi SHINDO

doi:10.1271/bbb.130148

Organic Chemistry Notes

Production of Hydroxlated Flavonoids with Cytochrome P450 BM3 Variant F87V and Their Antioxidative Activities

Emi KITAMURA, Toshihiko OTOMATSU, Chiemi MAEDA, Yoko AOKI, Chihiro OTA, Norihiko MISAWA, Kazutoshi SHINDO

著者情報

キーワード: flavonoid, P450 BM3, peptidyl-prolyl cis-trans isomerase, Escherichia coli, antioxidative activity

ジャーナルフリー

2013 年 77 巻 6 号 p. 1340-1343

DOI https://doi.org/10.1271/bbb.130148

View "Advance Publication" version (June 7, 2013).

詳細

抄録

A variant of P450 BM3 with an F87V substitution [P450 BM3 (F87V)] is a substrate-promiscuous cytochrome P450 monooxygenase. We investigated the bioconversion of various flavonoids (favanones, chalcone, and isoflavone) by using recombinant Escherichia coli cells, which expressed the gene coding for P450 BM3 (F87V), to give their corresponding hydroxylated products. Potent antioxidative activities were observed in some of the products.

責任著者(Corresponding author)

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