The UDP-N-acetylglucosamine:Dolichol Phosphate-N-acetylglucosamine-phosphotransferase Gene as a New Selection Marker for Potato Transformation

Hyun-Soon KIM; Mi-Sun KIM; Kyoung-A HWANG; Se-Won PARK; Jae-Heung JEON

doi:10.1271/bbb.130146

Biochemistry & Molecular Biology Notes

The UDP-N-acetylglucosamine:Dolichol Phosphate-N-acetylglucosamine-phosphotransferase Gene as a New Selection Marker for Potato Transformation

Hyun-Soon KIM, Mi-Sun KIM, Kyoung-A HWANG, Se-Won PARK, Jae-Heung JEON

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Keywords: Solanum tuberosum, UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine-phosphotransferase (GPT), selection marker gene, tunicamycin, transgenic plants

JOURNAL FREE ACCESS

2013 Volume 77 Issue 7 Pages 1589-1592

DOI https://doi.org/10.1271/bbb.130146

View "Advance Publication" version (July 7, 2013).

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Abstract

Here we describe the generation of potato plants that constitutively overexpressed, UDP-N-acetylglucosamine:dolichol phosphate-N-acetylglucosamine-phosphotransferase (GPT). Such transgenic plants can be formed in a medium with tunicamycin at 9.8 ± 0.28% efficiency, similar to the 9.4 ± 1.10 for the bialaphos resistance gene (Bar) gene. This study indicated that GPT transformation was very stable with high reproducibility, and that growth and tuber production in the GPT-transformed plants were stronger than in the wild-type plants.

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