A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species

Zewei AN; Yachao LI; Lili XIE; Qilin ZHAI; Huasun HUANG

doi:10.1271/bbb.130187

Biochemistry & Molecular Biology Notes

A Rapid and Economical Method for Low Molecular Weight RNA Isolation from a Wide Variety of Plant Species

Zewei AN, Yachao LI, Lili XIE, Qilin ZHAI, Huasun HUANG

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Keywords: low molecular weight RNA, microRNA, polyethylene glycol, plant

JOURNAL FREE ACCESS

2013 Volume 77 Issue 7 Pages 1599-1601

DOI https://doi.org/10.1271/bbb.130187

View "Advance Publication" version (July 7, 2013).

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Abstract

We have developed a time- and cost-effective method for isolating low molecular weight (LMW) RNA from plants. In our protocol, the isolation procedure can be completed within 3 h. Polyethylene glycol (PEG) and absolute ethanol are used to isolate LMW RNA, and the LMW RNA yields were >80 µg/g of fresh-weight tissues for several of the plant species tested.

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