Identification of Putative Biomarkers for Toluene-Degrading Burkholderia and Pseudomonads by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and Peptide Mass Fingerprinting

Erica M. HARTMANN; David R. COLQUHOUN; Rolf U. HALDEN

doi:10.1271/bbb.100064

This article has now been updated. Please use the final version.

Identification of Putative Biomarkers for Toluene-Degrading Burkholderia and Pseudomonads by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry and Peptide Mass Fingerprinting

Erica M. HARTMANN, David R. COLQUHOUN, Rolf U. HALDEN

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Keywords: proteomics, matrix-assisted laser desorption/ionization (MALDI), sodium dodecyl sulfate polyacrylimide gel electrophoresis (SDS PAGE), bioremediation, toluene

JOURNAL FREE ACCESS Advance online publication

Article ID: 100064

DOI https://doi.org/10.1271/bbb.100064

The final version of this article is now available: Vol. 74 (2010) , No. 7 p.1470

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Abstract

The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.

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