A Real-Time SUMO-Binding Assay for the Analysis of the SUMO-SIM Protein Interaction Network

Niina TANAKA; Hisato SAITOH

doi:10.1271/bbb.100082

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A Real-Time SUMO-Binding Assay for the Analysis of the SUMO-SIM Protein Interaction Network

Niina TANAKA, Hisato SAITOH

著者情報

キーワード: post-translational modification, SUMO, protein interaction, histone methyltransferase, chromatin

ジャーナルフリー早期公開

論文ID: 100082

DOI https://doi.org/10.1271/bbb.100082

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 6 p.1302

詳細

抄録

SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.

責任著者(Corresponding author)

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