Gene Sequencing and Tissue Expression of Unknown Isoforms of an Angiotensin II Type 2 Receptor Interacting Protein, ATIP, in the Rat

Michael Angus KREZEL; Linda Adriana REZMANN; Naghmeh VARGHAYEE; Josefa PETE; Albert George FRAUMAN; Simon Nicholas Stewart LOUIS

doi:10.1271/bbb.100176

This article has now been updated. Please use the final version.

Gene Sequencing and Tissue Expression of Unknown Isoforms of an Angiotensin II Type 2 Receptor Interacting Protein, ATIP, in the Rat

Michael Angus KREZEL, Linda Adriana REZMANN, Naghmeh VARGHAYEE, Josefa PETE, Albert George FRAUMAN, Simon Nicholas Stewart LOUIS

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Keywords: angiotensin II type 2 receptor interacting protein, gene expression, quantitative PCR, rat, sequencing

JOURNAL FREE ACCESS Advance online publication
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Article ID: 100176

DOI https://doi.org/10.1271/bbb.100176

The final version of this article is now available: Vol. 75 (2011) , No. 3 p.414

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Abstract

To investigate the expression of the unknown angiotensin II type 2 receptor interacting protein (ATIP) isoforms in the rat we used the known sequences of human and mouse ATIP to design sequencing primers to enable us to sequence rat ATIP3 and ATIP4. Exon 4, which is present in human but not mouse ATIP, was not identified in the coding region of rat ATIP. The expression levels of these genes in a range of rat tissues were examined, and we concluded that there is little similarity in the relative tissue distribution of the various ATIP isoforms in rat and human.

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