Functional Analysis of the Carbohydrate-Binding Module of an Esterase from Neisseria sicca SB Involved in the Degradation of Cellulose Acetate

Kunihiko MORIYOSHI; Daisuke KOMA; Hayato YAMANAKA; Takashi OHMOTO; Kiyofumi SAKAI

doi:10.1271/bbb.100213

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Functional Analysis of the Carbohydrate-Binding Module of an Esterase from Neisseria sicca SB Involved in the Degradation of Cellulose Acetate

Kunihiko MORIYOSHI, Daisuke KOMA, Hayato YAMANAKA, Takashi OHMOTO, Kiyofumi SAKAI

著者情報

キーワード: cellulose acetate, biodegradation, esterase, endo-1,4-β-glucanase, Neisseria sicca

ジャーナルフリー早期公開

論文ID: 100213

DOI https://doi.org/10.1271/bbb.100213

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 9 p.1940

詳細

抄録

An esterase gene from Neisseria sicca SB encoding CaeA, which catalyzes the deacetylation of cellulose acetate, was cloned. CaeA contained a putative catalytic domain of carbohydrate esterase family 1 and a carbohydrate-binding module (CBM) family 2. We constructed two derivatives, with and without the CBM of CaeA. Binding assay indicated that the CBM of CaeA had an affinity for cellulose.

責任著者(Corresponding author)

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