A New Beneficial Mutation in Pseudomonas sp. 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template

Fumi SHOZUI; Jian SUN; Yuyang SONG; Miwa YAMADA; Kohei SAKAI; Ken’ichiro MATSUMOTO; Kazuma TAKASE; Seiichi TAGUCHI

doi:10.1271/bbb.100224

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A New Beneficial Mutation in Pseudomonas sp. 61-3 Polyhydroxyalkanoate (PHA) Synthase for Enhanced Cellular Content of 3-Hydroxybutyrate-Based PHA Explored Using Its Enzyme Homolog as a Mutation Template

Fumi SHOZUI, Jian SUN, Yuyang SONG, Miwa YAMADA, Kohei SAKAI, Ken’ichiro MATSUMOTO, Kazuma TAKASE, Seiichi TAGUCHI

著者情報

キーワード: poly(3-hydroxybutyrate-co-3-hydroxyalkanoate), evolutionary engineering, PhaC1_Ps, 3-hydroxybutyrate-based copolymer

ジャーナルフリー早期公開

論文ID: 100224

DOI https://doi.org/10.1271/bbb.100224

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 8 p.1710

詳細

抄録

A newly isolated mutation (Gln508Leu) and a combination of it with previously discovered beneficial mutations in polyhydroxyalkanoate synthase 1 from Pseudomonas sp. 61-3 were found to enhance the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3HB-co-3-hydroxyalkanoate)s in recombinant Escherichia coli.

責任著者(Corresponding author)

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