Production of P(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) Terpolymers Using a Chimeric PHA Synthase in Recombinant Ralstonia eutropha and Pseudomonas putida

Jian SUN; Fumi SHOZUI; Miwa YAMADA; Ken’ichiro MATSUMOTO; Kazuma TAKASE; Seiichi TAGUCHI

doi:10.1271/bbb.100242

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Production of P(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) Terpolymers Using a Chimeric PHA Synthase in Recombinant Ralstonia eutropha and Pseudomonas putida

Jian SUN, Fumi SHOZUI, Miwa YAMADA, Ken’ichiro MATSUMOTO, Kazuma TAKASE, Seiichi TAGUCHI

著者情報

キーワード: chimeric enzyme, polyhydroxyalkanoate, PHA synthase, Pseudomonas putida, Aeromonas caviae

ジャーナルフリー早期公開

論文ID: 100242

DOI https://doi.org/10.1271/bbb.100242

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 8 p.1716

詳細

抄録

Recombinant strains of Ralstonia eutropha and Pseudomonas putida harboring a chimeric polyhydroxyalkanoate (PHA) synthase that consisted of PHA synthases of Aeromonas caviae and R. eutropha produced 3-hydroxybutyrate (3HB)-based PHA copolymers comprised of 3-hydroxyhexanoate and 3-hydroxyoctanoate units from dodecanoate (87–97 mol % 3HB), indicating that the chimeric PHA synthase possesses desirable substrate specificity leading to the production of 3HB-rich copolymers.

責任著者(Corresponding author)

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