Agrobacterium tumefaciens-Mediated Transformation of the Vegetative Dikaryotic Mycelium of the Cultivated Mushroom Flammulina velutipes

Tomomitsu OKAMOTO; Masato YAMADA; Shuichi SEKIYA; Toru OKUHARA; Goro TAGUCHI; Satoshi INATOMI; Makoto SHIMOSAKA

doi:10.1271/bbb.100398

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Agrobacterium tumefaciens-Mediated Transformation of the Vegetative Dikaryotic Mycelium of the Cultivated Mushroom Flammulina velutipes

Tomomitsu OKAMOTO, Masato YAMADA, Shuichi SEKIYA, Toru OKUHARA, Goro TAGUCHI, Satoshi INATOMI, Makoto SHIMOSAKA

著者情報

キーワード: Agrobacterium tumefaciens-mediated transformation, basidiomycete, Flammulina velutipes

ジャーナルフリー早期公開

論文ID: 100398

DOI https://doi.org/10.1271/bbb.100398

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 11 p.2327

詳細

抄録

Agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of Flammulina velutipes using a hygromycin B resistance gene as selectable marker. The gene coding for urogen III methyltransferase (cob) was introduced into F. velutipes dikaryotic cells. The resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in F. velutipes.

責任著者(Corresponding author)

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