Improvement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter

Tomoko MAEHARA; Shizuko TOMITA; Koji TAKABATAKE; Satoshi KANEKO

doi:10.1271/bbb.100556

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Improvement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter

Tomoko MAEHARA, Shizuko TOMITA, Koji TAKABATAKE, Satoshi KANEKO

著者情報

キーワード: Flammulina velutipes Fv-1, transformation, glyceraldehyde-3-phosphate dehydrogenase gene, promoter

ジャーナルフリー早期公開

論文ID: 100556

DOI https://doi.org/10.1271/bbb.100556

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 12 p.2523

詳細

抄録

To improve the expression level of heterologous genes in Flammulina velutipes Fv-1, we constructed new vectors having glyceraldehydes-3-phosphate dehydrogenase (gpd) gene promoter to control the expression of target genes. When the hygromycin B phosphotransferase (hph) gene from Escherichia coli was controlled by the gpd promoter, transformation efficiency was 3-fold higher than the case of that controlled by the tryptophan synthetase gene (trp1) promoter.

責任著者(Corresponding author)

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