A New Pathway for Poly(3-hydroxybutyrate) Production in Escherichia coli and Corynebacterium glutamicum by Functional Expression of a New Acetoacetyl-coenzyme A Synthase

Ken’ichiro MATSUMOTO; Miwa YAMADA; Chean Ring LEONG; Sung-Jin JO; Tomohisa KUZUYAMA; Seiichi TAGUCHI

doi:10.1271/bbb.100682

This article has now been updated. Please use the final version.

A New Pathway for Poly(3-hydroxybutyrate) Production in Escherichia coli and Corynebacterium glutamicum by Functional Expression of a New Acetoacetyl-coenzyme A Synthase

Ken’ichiro MATSUMOTO, Miwa YAMADA, Chean Ring LEONG, Sung-Jin JO, Tomohisa KUZUYAMA, Seiichi TAGUCHI

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Keywords: acetoacetyl-coenzyme A (CoA) synthesizing enzyme, acetyl-CoA, biodegradable plastics, biopolyester, malonyl-CoA

JOURNAL FREE ACCESS Advance online publication

Article ID: 100682

DOI https://doi.org/10.1271/bbb.100682

The final version of this article is now available: Vol. 75 (2011) , No. 2 p.364

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Abstract

A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] was developed in Escherichia coli and Corynebacterium glutamicum by an acetoacetyl-coenzyme A (CoA) synthase (AACS) recently isolated from terpenoid-producing Streptomyces sp. strain CL190. Expression of AACS led to significant productions of P(3HB) in E. coli (10.5 wt %) and C. glutamicum (19.7 wt %).

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