Heterologous Expression, Purification, and Characterization of an α-Mannosidase Belonging to Glycoside Hydrolase Family 99 of Shewanella amazonensis

Kana MATSUDA; Yuma KURAKATA; Takatsugu MIYAZAKI; Ichiro MATSUO; Yukishige ITO; Atsushi NISHIKAWA; Takashi TONOZUKA

doi:10.1271/bbb.100874

This article has now been updated. Please use the final version.

Heterologous Expression, Purification, and Characterization of an α-Mannosidase Belonging to Glycoside Hydrolase Family 99 of Shewanella amazonensis

Kana MATSUDA, Yuma KURAKATA, Takatsugu MIYAZAKI, Ichiro MATSUO, Yukishige ITO, Atsushi NISHIKAWA, Takashi TONOZUKA

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Keywords: α-mannosidase, Shewanella amazonensis, glycoside hydrolase family 99, N-glycosylation, pyridylamino sugar

JOURNAL FREE ACCESS Advance online publication

Article ID: 100874

DOI https://doi.org/10.1271/bbb.100874

The final version of this article is now available: Vol. 75 (2011) , No. 4 p.797

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Abstract

Shewanella amazonensis α-mannosidase (Sama99), a member of glycoside hydrolase family 99, was expressed in Escherichia coli. The purified Sama99 hydrolyzed pyridylamino (PA)-sugars, Glc₁Man₉GlcNAc₂-PA, and Glc₃Man₉GlcNAc₂-PA, and the product was probably a pyridylamino-decasaccharide in both cases. The mode of action of Sama99 was found to be essentially identical to that of rat endo-α-1,2-mannosidase, but the specificity of Sama99 was low.

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