Comparison of the Thermal Stabilities of the αβ Heterodimer and the α Subunit of Avian Myeloblastosis Virus Reverse Transcriptase

Atsushi KONISHI; Daisuke NEMOTO; Kiyoshi YASUKAWA; Kuniyo INOUYE

doi:10.1271/bbb.110238

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Comparison of the Thermal Stabilities of the αβ Heterodimer and the α Subunit of Avian Myeloblastosis Virus Reverse Transcriptase

Atsushi KONISHI, Daisuke NEMOTO, Kiyoshi YASUKAWA, Kuniyo INOUYE

著者情報

キーワード: avian myeloblastosis virus, insect cells, reverse transcriptase, template-primer, thermal stability

ジャーナルフリー早期公開

論文ID: 110238

DOI https://doi.org/10.1271/bbb.110238

この記事には本公開記事があります。

The final version of this article is now available: Vol. 75 (2011) , No. 8 p.1618

詳細

抄録

Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63-kDa α subunit and a 95-kDa β subunit. In this study, we explored the role of the interaction between the α and β subunits on AMV RT stability. The recombinant AMV RT α subunit was expressed in insect cells and purified. It exhibited lower thermal stability than the native AMV RT αβ heterodimer. Unlike the αβ heterodimer, the α subunit was not stabilized by template-primer. These results suggest that interaction between the α and β subunits is important for AMV RT stability.

責任著者(Corresponding author)

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