Characterization of a Loss-of-Function Mutant of Gibberellin Biosynthetic Gene LsGA3ox1 in Lettuce

Asami UMETSU; Yoshiaki SAWADA; Wataru MITSUHASHI; Marianne MAZIER; Tomonobu TOYOMASU

doi:10.1271/bbb.110475

This article has now been updated. Please use the final version.

Characterization of a Loss-of-Function Mutant of Gibberellin Biosynthetic Gene LsGA3ox1 in Lettuce

Asami UMETSU, Yoshiaki SAWADA, Wataru MITSUHASHI, Marianne MAZIER, Tomonobu TOYOMASU

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Keywords: dwarf, gibberellin biosynthesis, lettuce, loss-of-function mutant, retrotransposon

JOURNAL FREE ACCESS Advance online publication

Article ID: 110475

DOI https://doi.org/10.1271/bbb.110475

The final version of this article is now available: Vol. 75 (2011) , No. 12 p.2398

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Abstract

A previous study generated lettuce (Lactuca sativa) mutant lines tagged by retrotransposon Tnt1 from tobacco (Nicotiana tabacum) and identified a homozygous mutant, Tnt6a, that exhibited severe dwarf phenotype. Here we show that Tnt1 is inserted into the intron of gibberellin biosynthetic gene LsGA3ox1 in Tnt6a mutants. Expression analysis suggests that LsGA3ox1 is nearly knocked out in the Tnt6a mutants.

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