Screening of Optimal Cellulases from Symbiotic Protists of Termites through Expression in the Secretory Pathway of Saccharomyces cerevisiae

Nemuri TODAKA; Risa NAKAMURA; Sigeharu MORIYA; Moriya OHKUMA; Toshiaki KUDO; Haruo TAKAHASHI; Nobuhiro ISHIDA

doi:10.1271/bbb.110490

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Screening of Optimal Cellulases from Symbiotic Protists of Termites through Expression in the Secretory Pathway of Saccharomyces cerevisiae

Nemuri TODAKA, Risa NAKAMURA, Sigeharu MORIYA, Moriya OHKUMA, Toshiaki KUDO, Haruo TAKAHASHI, Nobuhiro ISHIDA

著者情報

キーワード: cellulase, termite, symbiotic protists, Saccharomyces cerevisiae

ジャーナルフリー早期公開

論文ID: 110490

DOI https://doi.org/10.1271/bbb.110490

この記事には本公開記事があります。

The final version of this article is now available: Vol. 75 (2011) , No. 11 p.2260

詳細

抄録

For direct and efficient ethanol production from cellulosic materials, we screened optimal cellulases from symbiotic protists of termites through heterologous expression with Saccharomyces cerevisiae. 11 cellulases, belonging to glycoside hydrolase families 5, 7, and 45 endoglucanases (EGs), were confirmed to produce with S. cerevisiae for the first time. A recombinant yeast expressing SM2042B24 EG I was more efficient at degrading carboxylmethyl cellulose than was Trichoderma reesei EG I, a major EG with high cellulolytic activity.

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