A Rapid and Simple Method of Evaluating the Dimeric Tendency of Fluorescent Proteins in Living Cells Using a Truncated Protein of Importin α as Fusion Tag

Chika NAKAGAWA; Shigenori NISHIMURA; Kaori SENDA-MURATA; Kenji SUGIMOTO

doi:10.1271/bbb.110677

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A Rapid and Simple Method of Evaluating the Dimeric Tendency of Fluorescent Proteins in Living Cells Using a Truncated Protein of Importin α as Fusion Tag

Chika NAKAGAWA, Shigenori NISHIMURA, Kaori SENDA-MURATA, Kenji SUGIMOTO

著者情報

キーワード: fluorescent protein (FP), Venus, weak dimer, importin alpha (importin α), nuclear-pore complex (NPC)

ジャーナルフリー早期公開

論文ID: 110677

DOI https://doi.org/10.1271/bbb.110677

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 2 p.388

詳細

抄録

Enhanced green fluorescent protein (EGFP) and its yellow variant (Venus) are weakly dimeric under physiological conditions. We designed a simple method to evaluate the dimeric tendency of fluorescent proteins in living mammalian cells. A novel single mutation, A206L, interfering with the hydrophobic interactions of the dimer interface in Venus, contributed to its monomerization, and was as effective as the A206K mutation in this assay.

責任著者(Corresponding author)

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