Flexible Plastic Bioreactors for Photobiological Hydrogen Production by Hydrogenase-Deficient Cyanobacteria

Masaharu KITASHIMA; Hajime MASUKAWA; Hidehiro SAKURAI; Kazuhito INOUE

doi:10.1271/bbb.110808

This article has now been updated. Please use the final version.

Flexible Plastic Bioreactors for Photobiological Hydrogen Production by Hydrogenase-Deficient Cyanobacteria

Masaharu KITASHIMA, Hajime MASUKAWA, Hidehiro SAKURAI, Kazuhito INOUE

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Keywords: bioreactor, cyanobacteria, hydrogen, hydrogen barrier film, photosynthesis

JOURNAL FREE ACCESS Advance online publication

Article ID: 110808

DOI https://doi.org/10.1271/bbb.110808

The final version of this article is now available: Vol. 76 (2012) , No. 4 p.831

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Abstract

Uptake hydrogenase mutant cells of the cyanobacterium Nostoc sp. PCC 7422 photobiologically produced H₂ catalyzed by nitrogenase for several days in H₂-barrier transparent plastic bags, and accumulated H₂ in the presence of O₂ evolved by photosynthesis. Their H₂ production activity was higher in the sealed flexible bags than in stoppered serum bottles of fixed gas volume.

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