Overexpression of the (R)-Specific Enoyl-CoA Hydratase Gene from Pseudomonas chlororaphis HS21 in Pseudomonas Strains for the Biosynthesis of Polyhydroxyalkanoates of Altered Monomer Composition

Moon Gyu CHUNG; Young Ha RHEE

doi:10.1271/bbb.110871

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Overexpression of the (R)-Specific Enoyl-CoA Hydratase Gene from Pseudomonas chlororaphis HS21 in Pseudomonas Strains for the Biosynthesis of Polyhydroxyalkanoates of Altered Monomer Composition

Moon Gyu CHUNG, Young Ha RHEE

著者情報

キーワード: monomer composition, PHA synthase, polyhydroxyalkanoates, Pseudomonas chlororaphis HS21, (R)-specific enoyl-CoA hydratase

ジャーナルフリー早期公開

論文ID: 110871

DOI https://doi.org/10.1271/bbb.110871

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 3 p.613

詳細

抄録

The (R)-specific enoyl-CoA hydratase gene (phaJ_HS21) from Pseudomonas chlororaphis HS21 was overexpressed in various Pseudomonas strains, alone and in combination with the polyhydroxyalkanoate synthase gene (phaC_HS21), for the biosynthesis of polyhydroxyalkanoates (PHAs) of altered monomer composition. Recombinant Pseudomonas strains harboring phaC_HS21 and phaJ_HS21 generated saturated and unsaturated monomers of C12–C14 in their PHAs. In particular, the level of the 3-hydroxytetradecenoate monomer in recombinant P. chlororaphis HS21 increased by approximately 260%. PhaJ_HS21 is expected to be useful in the biosynthesis of PHAs consisting of unusual monomer units.

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