Characterization of the C-Terminal Diglycine Motif of SUMO-1/3

Koji YAMADA; Miyuki MURAMATSU; Daiki SAITO; Mai SATO-OKA; Masayuki SAITO; Taishi MORIYAMA; Hisato SAITOH

doi:10.1271/bbb.120019

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Characterization of the C-Terminal Diglycine Motif of SUMO-1/3

Koji YAMADA, Miyuki MURAMATSU, Daiki SAITO, Mai SATO-OKA, Masayuki SAITO, Taishi MORIYAMA, Hisato SAITOH

著者情報

キーワード: small ubiquitin-related modifier (SUMO), ubiquitin, Ran GTPase activating protein (RanGAP1), post-translational modification

ジャーナルフリー早期公開

論文ID: 120019

DOI https://doi.org/10.1271/bbb.120019

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 5 p.1035

詳細

抄録

A hallmark of small ubiquitin-related modifier (SUMO) is the production of a C-terminal tail containing diglycines (GGs), which are believed to be required for SUMOylation. Whether GGs are required components in SUMOylation remains unanswered experimentally. In this study we found that the SUMO-1/3-AA/-GS/-GN/-GA mutant can form sodium dodecyl sulfate (SDS)-dithiothreitol (DTT)-resistant complexes with cellular proteins, indicating that the GG motif is not strictly required for SUMOylation.

責任著者(Corresponding author)

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