Mevalonate-Dependent Enzymatic Synthesis of Amorphadiene Driven by an ATP-Regeneration System Using Polyphosphate Kinase

Manami SHIMANE; Yoshinori SUGAI; Ryosuke KAINUMA; Masahiro NATSUME; Hiroshi KAWAIDE

doi:10.1271/bbb.120177

This article has now been updated. Please use the final version.

Mevalonate-Dependent Enzymatic Synthesis of Amorphadiene Driven by an ATP-Regeneration System Using Polyphosphate Kinase

Manami SHIMANE, Yoshinori SUGAI, Ryosuke KAINUMA, Masahiro NATSUME, Hiroshi KAWAIDE

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Keywords: amorphadiene, ATP-regeneration, mevalonate pathway, polyphosphate kinase, ³¹P-NMR

JOURNAL FREE ACCESS Advance online publication

Article ID: 120177

DOI https://doi.org/10.1271/bbb.120177

The final version of this article is now available: Vol. 76 (2012) , No. 8 p.1558

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Abstract

Polyphosphate kinase (PPK), which can regenerate ATP from ADP, was utilized in the mevalonate-dependent enzymatic synthesis of amorphadiene. The activity of PPK, cloned from Escherichia coli, was determined by ³¹P-NMR. The yield from the PPK-catalyzed synthesis was 25%, 2.5 times higher than that without PPK. The ³¹P-NMR analysis of the final reaction mixture indicated no accumulation of intermediates.

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