Immunostimulatory Activities of the Sulfated Polysaccharide Ascophyllan from Ascophyllum nodosum in in Vivo and in Vitro Systems

Kiyoaki NAKANO; Daekyung KIM; Zedong JIANG; Mikinori UENO; Takasi OKIMURA; Kenichi YAMAGUCHI; Tatsuya ODA

doi:10.1271/bbb.120232

This article has now been updated. Please use the final version.

Immunostimulatory Activities of the Sulfated Polysaccharide Ascophyllan from Ascophyllum nodosum in in Vivo and in Vitro Systems

Kiyoaki NAKANO, Daekyung KIM, Zedong JIANG, Mikinori UENO, Takasi OKIMURA, Kenichi YAMAGUCHI, Tatsuya ODA

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Keywords: Ascophyllum nodosum, ascophyllan, natural killer activity, macrophages, nitric oxide

JOURNAL FREE ACCESS Advance online publication

Article ID: 120232

DOI https://doi.org/10.1271/bbb.120232

The final version of this article is now available: Vol. 76 (2012) , No. 8 p.1573

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Abstract

Splenic natural killer (NK) cell activity against YAC-1 cells increased in mice intraperitoneally injected with ascophyllan. Ascophyllan enhanced the cytotoxicity of RAW264.7 cells toward YAC-1 cells in a concentration-dependent manner. The cytotoxicity of ascophyllan-stimulated RAW264.7 cells as to YAC-1 cells was suppressed with N^G-nitro-L-argininemethylester hydrochloride (L-NAME), an inhibitor of nitric oxide (NO) synthase, suggesting the involvement of NO in the cytotoxicity of ascophyllan-stimulated RAW264.7 cells.

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