抄録
Pyridylamino derivatization is suitable for the microanalysis of glycans but there is a problem in that by-products of the labeling reaction and fluorescent substances from samples occasionally interfere with the detection of pyridylamino glycans. We have reported a three-step purification method (S. Natsuka et al., FEBS J., 278, 452–460 (2011)). That method gives high purity and high yield for various glycans, but it is rather complicated. In this study I checked the efficiency of a one-step method with a spin column for the purification of pyridylamino glycans and found that it was excellent in respect of throughput. High-throughput analysis of N-glycans is desirable in glycomics.
