Cloning, Heterologous Expression, and Enzymatic Characterization of Cathepsin L from Starfish (Asterina pectinifera)

A Ram LEE; Hye Jin BAK; Na Young KIM; Moo-Sang KIM; Hye-Jin GO; Jin Woo HAN; Sang Jung AHN; Nam Gyu PARK; Joon Ki CHUNG; Hyung Ho LEE

doi:10.1271/bbb.120568

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Cloning, Heterologous Expression, and Enzymatic Characterization of Cathepsin L from Starfish (Asterina pectinifera)

A Ram LEE, Hye Jin BAK, Na Young KIM, Moo-Sang KIM, Hye-Jin GO, Jin Woo HAN, Sang Jung AHN, Nam Gyu PARK, Joon Ki CHUNG, Hyung Ho LEE

著者情報

キーワード: starfish, Asterina pectinifera, cathepsin L, recombinant protein, zymography

ジャーナルフリー早期公開

論文ID: 120568

DOI https://doi.org/10.1271/bbb.120568

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2012) , No. 12 p.2342

詳細

抄録

To determine the role of cathepsin L in Echinoderms, starfish (Asterina pectinifera) cathepsin L (ApCtL) was cloned. The results of RT-PCR analysis indicated that the expression of ApCtL in all of the tissues. The pro-mature enzyme of ApCtL, proApCtL, was expressed in Escherichia coli, and cathepsin activity was detected by cleaving of synthetic fluorogenic peptide substrates and gelatin zymography.

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