Isolation of the Putative Biosynthetic Gene Cluster of 1-Deoxynojirimycin by Bacillus amyloliquefaciens 140N, Its Production and Application to the Fermentation of Soybean Paste

Myung-Ji SEO; Young-Do NAM; So-Young LEE; So-Lim PARK; Sung-Hun YI; Seong-Il LIM

doi:10.1271/bbb.120753

This article has now been updated. Please use the final version.

Isolation of the Putative Biosynthetic Gene Cluster of 1-Deoxynojirimycin by Bacillus amyloliquefaciens 140N, Its Production and Application to the Fermentation of Soybean Paste

Myung-Ji SEO, Young-Do NAM, So-Young LEE, So-Lim PARK, Sung-Hun YI, Seong-Il LIM

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Keywords: Bacillus amyloliquefaciens, biosynthetic gene cluster, 1-deoxynojirimycin, fermented soybean paste, α-glucosidase inhibition

JOURNAL FREE ACCESS Advance online publication

Article ID: 120753

DOI https://doi.org/10.1271/bbb.120753

The final version of this article is now available: Vol. 77 (2013) , No. 2 p.398

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Abstract

The 1-deoxynojirimycin (DNJ) biosynthetic gene cluster of Bacillus amyloliquefaciens 140N isolated from traditional Korean fermented food was isolated by PCR screening. It showed 78.9% inhibitory activity against α-glucosidase and produced 0.8 g/L of DNJ in an optimized medium containing 2% soluble starch, 1% tryptone, 0.05% KH₂PO₄, and 0.05% (NH₂)₄SO₄. Soybean paste fermented with B. amyloliquefaciens 140N produced DNJ with 84.4% inhibitory activity.

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