Bioscience, Biotechnology, and Biochemistry
Online ISSN : 1347-6947
Print ISSN : 0916-8451

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Mandelate Racemase and Mandelate Dehydrogenase Coexpressed Recombinant Escherichia coli in the Synthesis of Benzoylformate
Dali LIZhen ZENGJunfang YANGPeng WANGLei JIANGJing FENGChengli YANG
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JOURNAL FREE ACCESS Advance online publication

Article ID: 121012

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Abstract
Benzoylformate is used widely as an organic synthetic intermediate. Mandelate racemase and mandelate dehydrogenase are two enzymes used in producing benzoylformate from racemic mandelate in microbial metabolism. In the present work, the genes encoding mandelate racemase and mandelate dehydrogenase from Pseudomanas aeruginosa strain NUST were cloned and expressed in Escherichia coli to convert racemic mandelate to benzoylformate. The use of whole resting cells of recombinant E. coli allowed the conversion of 65.7 mM solution of racemic mandelate to benzoylformate at a yield of 98.9% in 45 h. The process is a promising alternative for the production of benzoylformate.
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© 2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
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