Department of Bioengineering, Nanjing University of Science and Technology
Zhen ZENG
Department of Bioengineering, Nanjing University of Science and Technology
Junfang YANG
Department of Bioengineering, Nanjing University of Science and Technology
Peng WANG
Department of Bioengineering, Nanjing University of Science and Technology
Lei JIANG
Department of Bioengineering, Nanjing University of Science and Technology
Jing FENG
Department of Bioengineering, Nanjing University of Science and Technology
Chengli YANG
Department of Bioengineering, Nanjing University of Science and Technology Department of Microbiology, Key Laboratory for Microbiological Engineering of the Agricultural Environment of the Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University
Benzoylformate is used widely as an organic synthetic intermediate. Mandelate racemase and mandelate dehydrogenase are two enzymes used in producing benzoylformate from racemic mandelate in microbial metabolism. In the present work, the genes encoding mandelate racemase and mandelate dehydrogenase from Pseudomanas aeruginosa strain NUST were cloned and expressed in Escherichia coli to convert racemic mandelate to benzoylformate. The use of whole resting cells of recombinant E. coli allowed the conversion of 65.7 mM solution of racemic mandelate to benzoylformate at a yield of 98.9% in 45 h. The process is a promising alternative for the production of benzoylformate.
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