Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production

Osao ADACHI; Yoshitaka ANO; Hirohide TOYAMA; Kazunobu MATSUSHITA

doi:10.1271/bbb.60305

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Purification and Properties of NADP-Dependent Shikimate Dehydrogenase from Gluconobacter oxydans IFO 3244 and Its Application to Enzymatic Shikimate Production

Osao ADACHI, Yoshitaka ANO, Hirohide TOYAMA, Kazunobu MATSUSHITA

著者情報

キーワード: enzymatic shikimate production, Gluconobacter oxydans, osteltamivir, shikimate dehydrogenase

ジャーナルフリー早期公開

論文ID: 60305

DOI https://doi.org/10.1271/bbb.60305

この記事には本公開記事があります。

The final version of this article is now available: Vol. 70 (2006) , No. 11 p.2786

詳細

抄録

NADP-Dependent shikimate dehydrogenae (SKDH, EC 1.1.1.25) was purified from Gluconobacter oxydans IFO 3244. SKDH showed a single protein band on native-PAGE accompanying enzyme activity. It required NADP exclusively and catalyzed only the shuttle reaction between shikimate and 3-dehydroshikimate. The optimum pH for shikimate oxidation and 3-dehydroshikimate reduction was found at pH 10 and 7 respectively. SKDH proved to be a useful catalyst for shikimate production from 3-dehydroshikimate.

責任著者(Corresponding author)

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