Rhythmic Cycle of Clathrin-Coated Pit Formation at the trans-Golgi Network in Human MDA-MB-435 Cells

Shinji SAKAUSHI; Kaori SENDA-MURATA; Takashi FUKADA; Shigenori OKA; Kenji SUGIMOTO

doi:10.1271/bbb.60434

This article has now been updated. Please use the final version.

Rhythmic Cycle of Clathrin-Coated Pit Formation at the trans-Golgi Network in Human MDA-MB-435 Cells

Shinji SAKAUSHI, Kaori SENDA-MURATA, Takashi FUKADA, Shigenori OKA, Kenji SUGIMOTO

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Keywords: clathrin light chain a (CLCa), periodic fluctuations (PFs), live-imaging microscopy, brefeldin A (BFA), Golgi apparatus

JOURNAL FREE ACCESS Advance online publication
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Article ID: 60434

DOI https://doi.org/10.1271/bbb.60434

The final version of this article is now available: Vol. 71 (2007) , No. 2 p.571

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Abstract

We studied the in vivo dynamics of enhanced green fluorescent protein-tagged clathrin light chain a (GFP-CLCa) at the trans-Golgi network (TGN) in MDA-MB-435 cells. The intensity of fluorescence signals of GFP-CLCa periodically increased and decreased at the TGN approximately every 100 s. This suggests that the formation of clathrin-coated pits occurs synchronously and periodically at the TGN.

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