A New Method of Defense Response Analysis Using a Transient Expression System in Rice Protoplasts

Ryota TAKAI; Takashi KANEDA; Akira ISOGAI; Seiji TAKAYAMA; Fang-Sik CHE

doi:10.1271/bbb.60526

This article has now been updated. Please use the final version.

A New Method of Defense Response Analysis Using a Transient Expression System in Rice Protoplasts

Ryota TAKAI, Takashi KANEDA, Akira ISOGAI, Seiji TAKAYAMA, Fang-Sik CHE

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Keywords: protoplast, transient expression, rice, luciferase, defense response

JOURNAL FREE ACCESS Advance online publication

Article ID: 60526

DOI https://doi.org/10.1271/bbb.60526

The final version of this article is now available: Vol. 71 (2007) , No. 2 p.590

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Abstract

We established a new plant defense response assay using a transient expression system in rice protoplasts. The assay system sensitively detected defense induction by flagellin, which had previously been assigned to a specific elicitor. Our assay system provides a rapid and efficient way to dissect rice defense mechanisms.

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