Morphological Change in Cellular Granule Formation of Poly[(R)-3-hydroxybutyrate] Caused by DNA-Binding-Related Mutations of an Autoregulated Repressor PhaR

Abstract

PhaP is a major poly[(R)-3-hydroxybutyrate] [P(3HB)]-granule-associated protein. Its gene expression is controlled by an autoregulated repressor, PhaR, in Paracoccus denitrificans. The packing force of the P(3HB) granule by PhaP is greatly influenced by the number of PhaP molecules. In this study, the effect of a DNA-binding-ability-reduced mutation of PhaR on morphological change in the cellular granule formation of P(3HB) was examined under a transmission electron microscope using an Escherichia coli recombinant system. Microscopic observation indicated that stronger packing of P(3HB) granules took place when the number of PhaP molecules was increased by reduction in the DNA-binding ability of PhaR.