The Glucoamylase-Encoding Gene (glaB) Is Expressed in Solid-State Culture with A Low Water Content

Akiko KOBAYASHI; Motoaki SANO; Ken ODA; Hiromoto HISADA; Yoji HATA; Shinichi OHASHI

doi:10.1271/bbb.70132

This article has now been updated. Please use the final version.

The Glucoamylase-Encoding Gene (glaB) Is Expressed in Solid-State Culture with A Low Water Content

Akiko KOBAYASHI, Motoaki SANO, Ken ODA, Hiromoto HISADA, Yoji HATA, Shinichi OHASHI

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Keywords: Aspergillus oryzae, real-time PCR, solid-state culture, water content

JOURNAL FREE ACCESS Advance online publication

Article ID: 70132

DOI https://doi.org/10.1271/bbb.70132

The final version of this article is now available: Vol. 71 (2007) , No. 7 p.1797

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Abstract

Solid-state culture encourages high-level enzyme secretion by Aspergillus oryzae. Using the real-time quantitative reverse transcriptase-polymerase chain reaction, we confirmed that expression of the glucoamylase-encoding gene in A. oryzae cultured in solid-state culture depends on the water content of the culture.

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