Construction of a Yeast Strain with Regulatable Phospholipid Synthesis for Analysis of the Uptake and Metabolism of Phosphatidylethanolamine with Short Acyl Chains

Lan DENG; Toru KAKIHARA; Ryouichi FUKUDA; Akinori OHTA

doi:10.1271/bbb.70224

This article has now been updated. Please use the final version.

Construction of a Yeast Strain with Regulatable Phospholipid Synthesis for Analysis of the Uptake and Metabolism of Phosphatidylethanolamine with Short Acyl Chains

Lan DENG, Toru KAKIHARA, Ryouichi FUKUDA, Akinori OHTA

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Keywords: Saccharomyces cerevisiae, phosphatidylethanolamine, remodeling, transport

JOURNAL FREE ACCESS Advance online publication

Article ID: 70224

DOI https://doi.org/10.1271/bbb.70224

The final version of this article is now available: Vol. 71 (2007) , No. 9 p.2313

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Abstract

We constructed a yeast stain, TKY12Ga, in which phosphatidylethanolamine (PE) synthesis can be controlled by means of the carbon source in the medium. When PE synthesis was blocked, its growth was inhibited. However, in the presence of exogenous didecanoyl PE (diC10PE), TKY12Ga grew despite an inability to synthesize PE. Our system, which employs TKY12Ga strain and diC10PE, provides a valuable tool to study the transport and metabolism of PE in yeast.

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