Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster

Hye-Jin JUNG; Sung-Hee PARK; Sang-Do HA; Kyu-Ho LEE; Duck Hwa CHUNG; Cheol-Ho KIM; Min-Gon KIM; Kwang-Yup KIM; Keun-Sung KIM

doi:10.1271/bbb.70252

This article has now been updated. Please use the final version.

Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster

Hye-Jin JUNG, Sung-Hee PARK, Sang-Do HA, Kyu-Ho LEE, Duck Hwa CHUNG, Cheol-Ho KIM, Min-Gon KIM, Kwang-Yup KIM, Keun-Sung KIM

Author information

Hye-Jin JUNG
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
Sung-Hee PARK
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
Sang-Do HA
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University
Kyu-Ho LEE
Department of Environmental Engineering and Biotechnology, Hankuk University of Foreign Studies
Duck Hwa CHUNG
Division of Applied Life Science, Gyeongsang National University
Cheol-Ho KIM
Department of Biological Sciences, College of Natural Science, Sungkyunkwan University
Min-Gon KIM
Laboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology
Kwang-Yup KIM
Department of Food Science and Technology, Chungbuk National University
Keun-Sung KIM
Department of Food Science and Technology, College of Industrial Sciences, Chung-Ang University

Keywords: Listeria monocytogenes, PCR, prfA virulence gene cluster

JOURNAL FREE ACCESS Advance online publication

Article ID: 70252

DOI https://doi.org/10.1271/bbb.70252

The final version of this article is now available: Vol. 73 (2009) , No. 6 p.1412

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Abstract

Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.

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