Deletion Analysis of the Catalase-Encoding Gene (catB) Promoter from Aspergillus oryzae

抄録

The catalase-encoding gene (catB) is expressed strongly in Aspergillus oryzae. To identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using β-glucuronidase (GUS) as a reporter and an electrophoretic gel mobility shift assay (EMSA) systematically. The deletion 200-bp sequence from −1,000 to −800 in the 1,400-bp catB promoter caused a drastic decrease in GUS activity. In addition, EMSA implicated a 45-bp element from −1,000 to −956 containing cis-elements. According to detailed promoter deletion analysis, a region from −1,000 to −975, which contains putative heat shock element (HSE) and the CCAAT-box, was involved in strong expression.