Purification and Characterization of β-Glucosidase Involved in the Emission of 2-Phenylethanol from Rose Flowers

Miwa SAKAI; Saori TOMITA; Hiroshi HIRATA; Tatsuo ASAI; Hideo DOHRA; Masakazu HARA; Naoharu WATANABE

doi:10.1271/bbb.70404

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Purification and Characterization of β-Glucosidase Involved in the Emission of 2-Phenylethanol from Rose Flowers

Miwa SAKAI, Saori TOMITA, Hiroshi HIRATA, Tatsuo ASAI, Hideo DOHRA, Masakazu HARA, Naoharu WATANABE

著者情報

キーワード: β-glucosidase, Rosa ‘Hoh-Jun’, petal, 2-phenylethyl β-D-glucopyranoside, blue native-PAGE

ジャーナルフリー早期公開

論文ID: 70404

DOI https://doi.org/10.1271/bbb.70404

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 1 p.219

詳細

抄録

β-Glucosidase was partially purified from Rosa ‘Hoh-Jun’ petals. The enzyme was highly specific for such β-D-glucopyranosides as 2-phenylethyl β-D-glucopyranoside. The optimal activity was observed at pH 6.0 and 35 °C. The enzymes were composed with two proteins (160 and 155 kDa) by blue native-PAGE, and were classified in a family 1 glucosidase based on LC-MS/MS analyses.

責任著者(Corresponding author)

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