Expression and DNA Binding Activity of the Tomato Transcription Factor RIN (Ripening Inhibitor)

Hong-Liang ZHU; Ben-Zhong ZHU; Ying-Cong LI; Yi SHAO; Xiao-Guang WANG; Yuan-Hong XIE; An-Jun CHEN; Jun-Jie LUO; Xiao-Yan JIA; Yun-Bo LUO

doi:10.1271/bbb.70577

This article has now been updated. Please use the final version.

Expression and DNA Binding Activity of the Tomato Transcription Factor RIN (Ripening Inhibitor)

Hong-Liang ZHU, Ben-Zhong ZHU, Ying-Cong LI, Yi SHAO, Xiao-Guang WANG, Yuan-Hong XIE, An-Jun CHEN, Jun-Jie LUO, Xiao-Yan JIA, Yun-Bo LUO

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Keywords: binding activity, electrophoretic mobility shift assay (EMSA), Mcm1, Agamous, Deficiens, Srf (MADS)-box, ripening inhibitor, tomato

JOURNAL FREE ACCESS Advance online publication

Article ID: 70577

DOI https://doi.org/10.1271/bbb.70577

The final version of this article is now available: Vol. 72 (2008) , No. 1 p.250

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Abstract

Recently, we have found that the accumulation of ripening inhibitor (RIN) protein increased gradually during tomato fruit ripening. Here, the recombinant protein was expressed in Escherichia coli and affinity-purified. The DNA binding activity of renatured RIN protein was tested by electrophoretic mobility shift assay. The results indicated that an optimal expression and purification system was suitable for obtaining active RIN with DNA binding activity.

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