Construction of a Binary Vector for Knockout and Expression Analysis of Rice Blast Fungus Genes

Ken-ichiro SAITOH; Marie NISHIMURA; Yasuyuki KUBO; Nagao HAYASHI; Eiichi MINAMI; Yoko NISHIZAWA

doi:10.1271/bbb.70834

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Construction of a Binary Vector for Knockout and Expression Analysis of Rice Blast Fungus Genes

Ken-ichiro SAITOH, Marie NISHIMURA, Yasuyuki KUBO, Nagao HAYASHI, Eiichi MINAMI, Yoko NISHIZAWA

著者情報

キーワード: gene knockout, green fluorescent protein (GFP)-tagging, gene expression imaging, Agrobacterium-mediated transformation, Magnaporthe grisea

ジャーナルフリー早期公開

論文ID: 70834

DOI https://doi.org/10.1271/bbb.70834

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 5 p.1380

詳細

抄録

We developed an efficient method to analyze gene function and expression of the rice blast fungus. We constructed a GATEWAY binary vector, which generates a gene-targeted disruptant carrying a green fluorescent protein gene under the native promoter of the target gene. Using this method, the knockout efficiency and expression patterns of two hypothetical genes were determined.

責任著者(Corresponding author)

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