Determination of the Luciferin Contents in Luminous and Non-Luminous Beetles

Yuichi OBA; Takeru SHINTANI; Takanori NAKAMURA; Makoto OJIKA; Satoshi INOUYE

doi:10.1271/bbb.80017

This article has now been updated. Please use the final version.

Determination of the Luciferin Contents in Luminous and Non-Luminous Beetles

Yuichi OBA, Takeru SHINTANI, Takanori NAKAMURA, Makoto OJIKA, Satoshi INOUYE

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Keywords: bioluminescence, Cantharidae, Elateridae, Lampyridae, Lycidae

JOURNAL FREE ACCESS Advance online publication

Article ID: 80017

DOI https://doi.org/10.1271/bbb.80017

The final version of this article is now available: Vol. 72 (2008) , No. 5 p.1384

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Abstract

The contents of firefly luciferin in luminous and non-luminous beetles were determined by the methods of HPLC with fluorescence detection and the luminescence reaction of luciferin and firefly luciferase. Luminous cantharoids and elaterids contained various amounts of luciferin in the range of pmol to hundreds of nmol, but no luciferin was detected in the non-luminous cantharoids and elaterids.

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