Identification of Five Phospho-β-glycosidases from Lactobacillus gasseri ATCC33323T Cultured in Lactose Medium

Seiji NAGAOKA; Hiroyuki HONDA; Satoru OHSHIMA; Yasushi KAWAI; Haruki KITAZAWA; Yoshio TATENO; Yukiko YAMAZAKI; Tadao SAITO

doi:10.1271/bbb.80089

This article has now been updated. Please use the final version.

Identification of Five Phospho-β-glycosidases from Lactobacillus gasseri ATCC33323^T Cultured in Lactose Medium

Seiji NAGAOKA, Hiroyuki HONDA, Satoru OHSHIMA, Yasushi KAWAI, Haruki KITAZAWA, Yoshio TATENO, Yukiko YAMAZAKI, Tadao SAITO

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Keywords: Lactobacillus gasseri, phospho-β-galactosidase, phospho-β-glucosidase, lactose

JOURNAL FREE ACCESS Advance online publication

Article ID: 80089

DOI https://doi.org/10.1271/bbb.80089

The final version of this article is now available: Vol. 72 (2008) , No. 7 p.1954

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Abstract

Lactobacillus gasseri ATCC33323^T has seven putative phospho-β-glycosidase genes. Using column chromatography, we found that this strain cultured in lactose medium expresses five phospho-β-glycosidases (LacG1, LacG2, Pbg1, Pbg2, and Pbg3), where these gene expressions can be suppressed by glucose. To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose.

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