Analysis of Inactivation of AcMNPV under Various Conditions by the ELVA Method

Takeshi GOTOH; Nozomi ANDO; Ken-Ichi KIKUCHI

doi:10.1271/bbb.80105

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Analysis of Inactivation of AcMNPV under Various Conditions by the ELVA Method

Takeshi GOTOH, Nozomi ANDO, Ken-Ichi KIKUCHI

著者情報

キーワード: baculoviruses, Autographa californica multiple nucleopolyhedrovirus (AcMNPV), inactivation, stability, envelope-labeled virus assay (ELVA)

ジャーナルフリー早期公開

論文ID: 80105

DOI https://doi.org/10.1271/bbb.80105

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 7 p.1973

詳細

抄録

Inactivation of baculoviruses was examined under various conditions by ELVA, which enzymatically labels the virus envelope with radioisotopes and detects the labeled viruses by host cell-specific binding. When baculoviruses were incubated in a 50-ml culture tube, they rapidly lost infective ability by more than 75% of the initial titer in 2 h even at an insect cell cultivation temperature of 27 °C. Altering pH from neutral significantly decreased the virus titer. Repetition of freezing and thawing of baculovirus solutions decreased the virus titer, but the addition of DMSO and glycerol prevented inactivation to some extent.

責任著者(Corresponding author)

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