Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)

Andian Ari ANGGRAENI; Makiko SAKKA; Tetsuya KIMURA; Khanok RATANAKHAOKCHAI; Motomitsu KITAOKA; Kazuo SAKKA

doi:10.1271/bbb.80242

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引用する場合も本公開記事を引用してください。

Characterization of Bacillus halodurans α-Galactosidase Mel4A Encoded by the mel4A Gene (BH2228)

Andian Ari ANGGRAENI, Makiko SAKKA, Tetsuya KIMURA, Khanok RATANAKHAOKCHAI, Motomitsu KITAOKA, Kazuo SAKKA

著者情報

キーワード: Bacillus halodurans, α-galactosidase, melibiase, glycoside hydrolase, raffinose

ジャーナルフリー早期公開

論文ID: 80242

DOI https://doi.org/10.1271/bbb.80242

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 9 p.2459

詳細

抄録

A family-4 α-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD⁺, some divalent cations such as Mn²⁺, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.

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