Selection of an Effective Red-Pigment Producing Monascus pilosus by Efficient Transformation with Aurintricarboxylic Acid

Yi-Pei CHEN; I-Ching CHEN; Ing-Er HWANG; Gwo-Fang YUAN; Li-Ling LIAW; Ching-Ping TSENG

doi:10.1271/bbb.80342

This article has now been updated. Please use the final version.

Selection of an Effective Red-Pigment Producing Monascus pilosus by Efficient Transformation with Aurintricarboxylic Acid

Yi-Pei CHEN, I-Ching CHEN, Ing-Er HWANG, Gwo-Fang YUAN, Li-Ling LIAW, Ching-Ping TSENG

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Keywords: Monascus pilosus, restriction-enzyme-mediated integration (REMI), aurintricarboxylic acid (ATA)

JOURNAL FREE ACCESS Advance online publication

Article ID: 80342

DOI https://doi.org/10.1271/bbb.80342

The final version of this article is now available: Vol. 72 (2008) , No. 11 p.3021

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Abstract

The filamentous fungus Monascus pilosus was genetically transformed with a reporter plasmid, pMS-1.5hp, by aurintricarboxylic acid (ATA) treatment to obtain an efficient red-pigment producing mutant. The transformation efficiency of Monascus pilosus was higher with the ATA-treatment than with either a non-restriction-enzyme-mediated integration (REMI) or a REMI method. This valid and convenient random mutagenesis method shows that ATA can be applied in fungi for efficient genetic transformation.

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