Prenyl Alcohol Production by Expression of Exogenous Isopentenyl Diphosphate Isomerase and Farnesyl Diphosphate Synthase Genes in Escherichia coli

Chikara OHTO; Masayoshi MURAMATSU; Shusei OBATA; Eiji SAKURADANI; Sakayu SHIMIZU

doi:10.1271/bbb.80446

This article has now been updated. Please use the final version.

Prenyl Alcohol Production by Expression of Exogenous Isopentenyl Diphosphate Isomerase and Farnesyl Diphosphate Synthase Genes in Escherichia coli

Chikara OHTO, Masayoshi MURAMATSU, Shusei OBATA, Eiji SAKURADANI, Sakayu SHIMIZU

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Keywords: farnesol, geranylgeraniol, lipoate, pyruvate, terpenoid

JOURNAL FREE ACCESS Advance online publication

Article ID: 80446

DOI https://doi.org/10.1271/bbb.80446

The final version of this article is now available: Vol. 73 (2009) , No. 1 p.186

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Abstract

Isopentenyl diphosphate isomerase (idi) and farnesyl diphosphate synthase (ispA) genes were overexpressed in Escherichia coli. The resulting transformant showed 6.8-fold higher production of farnesol (389 μg/l). In a similar manner, overexpression of idi and mutated ispA led to high production of geranylgeraniol (128 μg/l).

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